Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
Price: $ 180
Price: $ 120
- Sample Type: serum;plasma;animal tissue;cells;cell culture supernatant
- Detection Instrument: Microplate reader(500-520 nm)
- Reasearch Areas: Liver Renal function biomarkers
For research use only. Order now, ship in 3 days
Detection Principle | ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm. | ||||||||||||||||||
Synonyms | ALT;GPT;Glutamic-pyruvic transaminase | ||||||||||||||||||
Sample Type | serum,plasma,animal tissue,cells,cell culture supernatant | ||||||||||||||||||
Sensitivity | 0.75 IU/L | ||||||||||||||||||
Detection Range | 0.75-72.3 IU/L | ||||||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||||||
Assay Time | 80 min | ||||||||||||||||||
Precision | inter-assay CV: 9% | intra-assay CV: 5.3% | ||||||||||||||||||
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) | ||||||||||||||||||
Storage | This product can be stored at 2~8°C for 12 months with shading light. | ||||||||||||||||||
Valid Period | 12 months | ||||||||||||||||||
Images | |||||||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor. |
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Q1:The tissue sample has been frozen for a long time, can it be used to detect AST/ALT?
It is recommended to use fresh samples as far as possible. If fresh samples are not available, it is recommended that the samples be frozen at -80℃ for no more than one month, otherwise the AST/ALT enzyme activity will decrease, which will have a great impact on the results.