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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Cat:E-BC-K235-M Citations (68)
Manual MSDS

Price: $ 180

Price: $ 120

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;animal tissue;cells;cell culture supernatant
  • Detection Instrument: Microplate reader(500-520 nm)
  • Reasearch Areas: Liver Renal function biomarkers
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Product Details
Detection Principle ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Synonyms ALT;GPT;Glutamic-pyruvic transaminase
Sample Type serum,plasma,animal tissue,cells,cell culture supernatant
Sensitivity 0.75 IU/L
Detection Range 0.75-72.3 IU/L
Detection Method Colorimetric method
Assay Time 80 min
Precision inter-assay CV: 9% | intra-assay CV: 5.3%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Human plasma 1
Porcine serum 1
Rat serum 1
10% Rat brain tissue homogenization 1
10% Rat heart tissue homogenization 1
10% Rat liver tissue homogenization 40-60
10% Rat kidney tissue homogenization 1
The diluent is saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
  • Q1:The tissue sample has been frozen for a long time, can it be used to detect AST/ALT?

    It is recommended to use fresh samples as far as possible. If fresh samples are not available, it is recommended that the samples be frozen at -80℃ for no more than one month, otherwise the AST/ALT enzyme activity will decrease, which will have a great impact on the results.