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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Name: Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
Brand: Elabscience
SKU: E-BC-K235-M
Price: 180 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(6)

Cat.No.:E-BC-K235-M
Detection instrument: Microplate reader(500-520 nm)

Detection method: Colorimetric method

Manual MSDS

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Detection principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Performance characteristics

Synonyms	ALT,GPT,Glutamic-pyruvic transaminase
Sample type	Serum,plasma,animal tissue,cells,cell culture supernatant
Sensitivity	0.75 IU/L
Detection range	0.75-72.3 IU/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	80 min
Precision	Average inter-assay CV: 9%Average intra-assay CV: 5.300%
Other instruments required	Micropipettor, Vortex mixer, Incubator, Multichannel pipette
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

No significant change of ALT activity was observed, the LCFG nanoparticles did not include hepatotoxicity.

X Zhang et al investigate the usage of H2O2 and O2 for cooperative chemodynamic/starvation cancer therapy. Alanine aminotransferase (ALT) avtivity in mouse blood was determined using ALT activity assay kit (E-BC-K235-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.75-72.3 IU/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Porcine serum	1
Rat serum	1
10% Rat brain tissue homogenization	1
10% Rat heart tissue homogenization	1
10% Rat liver tissue homogenization	40-60
10% Rat kidney tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Journal Of Hazardous Materials (2020) IF: 10.588

The combined adverse effects of cis-bifenthrin and graphene oxide on lipid homeostasis in Xenopus laevis

DOI: 10.1016/j.jhazmat.2020.124876
PMID: 33360192	Sample: Tissue homogenate

Food and Chemical Toxicology (2021) IF: 6.023

Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

DOI: 10.1016/j.fct.2021.112124
PMID: 33727180	Sample: Serum

FEBS Letters (2020) IF: 3.057

Islet-cell autoantigen 69 accelerates liver regeneration by downregulating Tgfbr1 and attenuating Tgfβ signaling in mice

DOI: 10.1002/1873-3468.13859
PMID: 32531799	Sample: Serum

ACS Applied Bio Materials (2020)

Noninvasive Visualization of Obesity-Boosted Inflammation in Orthotopic Pancreatic Ductal Adenocarcinoma Using an Octapod Iron Oxide Nanoparticle

DOI: 10.1021/acsabm.0c00841
	Sample: Serum

Journal of Physics: Conference Series (2019)

Hepatoprotective effect of Eugenol on Acetaminophen-Induced Hepatotoxicity in HepG2 cells

DOI: 10.1088/1742-6596/1374/1/012009
	Sample: Cell sample

Synergy (2018)

Omega-3 fatty acids moderate oxidative and proinflammatory events in experimental hepatotoxicity in Wistar rats: comparison with livolin

DOI: 10.1016/j.synres.2018.08.001
	Sample: Plasma

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Reviews/Q&A

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Q&A

Verified Customer

a****gSubmitted [ May 29 2019 ]

Application:Biochemical
Description:This alanine aminotransferase assay kit requires a small sample size, which is of great help to our real project.

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E**************zSubmitted [ May 15 2023 ]

Asked: We are currently using C57BL/6 strain and the maximum serum that we collect is only 30 uL. Can you tell me how much serum sample that I have to use for one experiment?

adminSubmitted [ May 15 2023 ]

Answered: The minimum requirement for a single well is 10 uL for kit E-BC-K235-M

M*****aSubmitted [ Aug 31 2022 ]

Asked: Will it affect result if this kit is at room temperature for half a month？

adminSubmitted [ Aug 31 2022 ]

Answered: No, it will not influence the result. Please ensure using.

M*****aSubmitted [ Aug 22 2022 ]

Asked: Can you please help me understand exactly what a carmen unit is and why we are using it instead of the conventional method of plotting a standard curve of concentration of standard vs the absorbance. How is the carmen unit relevant to this exact calculation I would also like to know how you arrived at the numbers 0, 28, 57, 97, 150, 200 for the standard Carmen values.

adminSubmitted [ Aug 22 2022 ]

Answered: 1. This indicator is special, and the method used is the Reitman-Frankel method. The standard curve of the Reitman-Frankel method is determined by comparing the colorimetric method experimental result with the result of karmen spectrophotometry (rate method), and the report result of Karmen units will be more accurate. 2. The Karmen's units corresponding to the standard are fixed and converted according to the concentration of pyruvate.

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