Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (E-BC-K235-M)
For research use only.
| Detection Principle | ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm. |
| Synonyms | ALT;GPT;Glutamic-pyruvic transaminase |
| Sample Type | Serum,Plasma,Animal tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-520 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.75 IU/L |
| Detection Range | 0.75-72.30 IU/L |
| Precision | inter-assay CV: 0.09 | intra-assay CV: 5.3% |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Porcine serum | 1 |
| Rat serum | 1 |
| 10% Rat brain tissue homogenization | 1 |
| 10% Rat heart tissue homogenization | 1 |
| 10% Rat liver tissue homogenization | 40-60 |
| 10% Rat kidney tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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