Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method)

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K235-M

      • 96T
      • 48T
      - +
      Price: $180
      Can detect 42 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(500-520 nm)

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      Detection principle

      ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

      Performance characteristics

      Synonyms ALT,GPT,Glutamic-pyruvic transaminase
      Sample type Serum,plasma,animal tissue,cells,cell culture supernatant
      Sensitivity 0.75 IU/L
      Detection range 0.75-72.3 IU/L
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 80 min
      Precision Average inter-assay CV: 9%Average intra-assay CV: 5.3%
      Other instruments required Micropipettor, Vortex mixer, Incubator, Multichannel pipette
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months


      1. Islet-cell autoantigen 69 accelerates liver regeneration by downregulating Tgfbr1 and attenuating Tgfβ signaling in mice
        IF: 3.057
        Journal: FEBS Letters(2020)
        DOI: 10.1002/1873-3468.13859
        PMID: 32531799
        Products: E-BC-K235-M E-EL-M0160 
        Sample type: Serum
        Reactivity: Mouse
      2. Noninvasive Visualization of Obesity-Boosted Inflammation in Orthotopic Pancreatic Ductal Adenocarcinoma Using an Octapod Iron Oxide Nanoparticle
        Journal: ACS Applied Bio Materials(2020)
        DOI: 10.1021/acsabm.0c00841
        PMID: 99999999
        Products: E-BC-K091-S E-BC-K235-M E-BC-K236-M 
        Sample type: Serum
        Reactivity: Mouse
      3. Hepatoprotective effect of Eugenol on Acetaminophen-Induced Hepatotoxicity in HepG2 cells
        Journal: Journal of Physics: Conference Series(2019)
        DOI: 10.1088/1742-6596/1374/1/012009
        PMID: 99999999
        Products: E-BC-K045 E-BC-K235-M E-BC-K236-M 
        Sample type: Cell sample
        Reactivity: Human
      4. Omega-3 fatty acids moderate oxidative and proinflammatory events in experimental hepatotoxicity in Wistar rats: comparison with livolin
        Journal: Synergy(2018)
        DOI: 10.1016/j.synres.2018.08.001
        PMID: 99999999
        Products: E-BC-K025-M E-BC-K031-M E-BC-K091-M E-BC-K096-M E-BC-K210 E-BC-K235-M E-BC-K236-M E-EL-0076 
        Sample type: Plasma
        Reactivity: Rat
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      Verified Customer

      a****gSubmitted [ May 29 2019 ]

      • Application:Biochemical
      • Description:This alanine aminotransferase assay kit requires a small sample size, which is of great help to our real project.
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      MSDS for ELISA ISO 9001

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