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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Cat.No.:E-BC-K235-S
Detection instrument: Spectrophotometer(505 nm)

Detection method: Colorimetric method

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Detection principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Performance characteristics

Synonyms	ALT,GPT,Glutamic-pyruvic transaminase
Sample type	Serum,plasma,cells,animal tissue
Sensitivity	1.26 IU/L
Detection range	1.26-72.3 IU/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	100 min
Precision	Average inter-assay CV: 8.200%Average intra-assay CV: 4.300%
Other instruments required	Micropipettor, Vortex mixer, Incubator
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.26-72.3 IU/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Rat liver tissue homogenization	30-60
10% Rat kidney tissue homogenization	1
Human serum	1
Human plasma	1
HepG2 cells homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Cells (2021) IF: 6.6
Indole-3-Carboxaldehyde Restores Gut Mucosal Integrity and Protects from Liver Fibrosis in Murine Sclerosing Cholangitis

DOI: 10.3390/cells10071622

PMID: 34209524
Sample: serum
PLoS Pathogens (2021) IF: 6.823
IRF3-mediated pathogenicity in a murine model of human hepatitis A

DOI: 10.1371/journal.ppat.1009960

PMID: 34591933
Sample: serum
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

DOI: 10.1016/j.jep.2022.115306

PMID: 35443217
Sample: serum
BMC Complementary and Alternative Medicine (2022) IF: 3.659
Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues

DOI: 10.1186/s12906-022-03536-x

PMID: 35277165
Sample: serum
PeerJ (2022) IF: 2.984
3,4-dihydroxyphenylethyl alcohol glycoside reduces acetaminophen-induced acute liver failure in mice by inhibiting hepatocyte ferroptosis and pyroptosis

DOI: 10.7717/peerj.13082

PMID: 35310165
Sample: plasma
Journal of Nanomaterials (2021) IF: 2.986
Effect of Hafnium Coating on Osseointegration of Titanium Implants: A Split Mouth Animal Study

DOI: 10.1155/2021/7512957

Sample: mandibular bone
TOXICOLOGY AND INDUSTRIAL HEALTH (2019) IF: 1.635
Toxic effects of subchronic oral acetamiprid exposure in rats:

DOI: 10.1177/0748233719893203

Sample: plasma
Toxicology Reports (2017)
Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

DOI: 10.1016/j.toxrep.2017.12.002

Sample: plasma

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