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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method)

  • Cat.No.:E-BC-K235-S

  • Detection instrument: Spectrophotometer(505 nm)

To Purchase E-BC-K235-S

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  • 100Assays
  • 50Assays
Price: $180
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Detection principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Performance characteristics

Synonyms ALT,GPT,Glutamic-pyruvic transaminase
Sample type Serum,plasma,cells,animal tissue
Sensitivity 1.26 IU/L
Detection range 1.26-72.3 IU/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 8.199999999999999%Average intra-assay CV: 4.3%
Other instruments required Micropipettor, Vortex mixer, Incubator
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.26-72.3 IU/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Rat liver tissue homogenization

30-60

10% Rat kidney tissue homogenization

1

Human serum

1

Human plasma

1

HepG2 cells homogenization

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

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