Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K235-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $180
      Can detect 44 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(505 nm)

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      Detection principle

      ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

      Performance characteristics

      Synonyms ALT,GPT,Glutamic-pyruvic transaminase
      Sample type Serum,plasma,cells,animal tissue
      Sensitivity 1.26 IU/L
      Detection range 1.26-72.3 IU/L
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 100 min
      Precision Average inter-assay CV: 8.2%Average intra-assay CV: 4.3%
      Other instruments required Micropipettor, Vortex mixer, Incubator
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months
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      MSDS for ELISA ISO 9001

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