Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method) (E-BC-K057-S)
For research use only.
| Detection Principle | Bromocresol green (BCG) can combine with the albumin in pH 4.0-4.2 to form an albumin-BCG complex, which is yellowish-green. The depth of yellowish-green is proportional to the concentration of albumin. The serum albumin concentration can be calculated by measuring the OD value at 628 nm. |
| Synonyms | ALB |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (628 nm) |
| Research Area | Liver And Renal Function , Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.50 g/L |
| Detection Range | 0.50-70 g/L |
| Precision | inter-assay CV: 4.2% | intra-assay CV: 2.1% |
| Sample Volume | 10 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse plasma | 1 |
| Rat serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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