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Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)

  • Cat.No.:E-BC-K057-S

  • Detection instrument: Spectrophotometer(628 nm)

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Price: $150

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Detection principle

Bromocresol green (BCG) can combine with the albumin in pH 4.0~4.2 to form an albumin-BCG complex, which is yellowish-green. The depth of yellowish-green is proportional to the concentration of albumin. The serum albumin concentration can be calculated by measuring the OD value at 628 nm.

Performance characteristics

Synonyms ALB
Sample type Serum,plasma
Sensitivity 0.50 g/L
Detection range 0.50-70 g/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 50 min
Precision Average inter-assay CV: 4.200%Average intra-assay CV: 2.100%
Other instruments required Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 1: 2-8℃, Reagent 2: -20℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.50-70 g/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Human plasma


Mouse plasma


Rat serum


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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    DOI: 10.3390/ijms232315400

    Sample: plasma,urine
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    DOI: 10.2147/DDDT.S326975

    PMID: 34703210

    Sample: serum
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    Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

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    Sample: plasma
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    DOI: 10.5650/jos.ess22033

    PMID: 35965085

    Sample: serum
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