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Aldo-Keto Reductase (ARKs) Activity Assay Kit

  • Cat.No.:E-BC-K810-M

  • Detection instrument: Microplate reader (450 nm)

To Purchase E-BC-K810-M

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  • 96T
Price: $480
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Detection principle

Aldo-keto reductase (AKRS) is distributed in prokaryotes and eukaryotes, and there are a variety of isoforms. AKRS can catalyze the NADPH-dependent carbonyl reduction reaction to generate the corresponding alcohols. Studies have shown that AKRS are associated with a variety of cancers. The principle of this kit is that the substrate is catalyzed by AKRS, and the chromogenic substance produced by the reaction of the product and the chromogenic agent has a characteristic absorption peak at 450 nm. By detecting the amount of chromogenic substance produced per unit time, the activity of AKRS enzyme in the sample can be calculated.

Performance characteristics

Synonyms ARKs
Sample type serum;plasma;tissue
Sensitivity 0.05 U/L
Detection range 0.05-45.00 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 50 min
Precision Average inter-assay CV: 7.1-10%Average intra-assay CV: 1.9-4.8%
Other instruments required Incubator (37℃)
Other reagents required Double distilled water, Normal saline (0.9% NaCl)
Storage -20℃
Valid period 12 months

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