Free Shipping 24T ELISA Kit
Synonyms:coronavirus s1,coronavirus s2,coronavirus spike,cov spike,ncov RBD,ncov s1,ncov s2,ncov spike,novel coronavirus RBD,novel coronavirus s1,novel coronavirus s2,novel coronavirus spike,RBD,S1,s2,Spike RBD
Applications: Neutralization, ELISA
|Synonyms||coronavirus s1,coronavirus s2,coronavirus spike,cov spike,ncov RBD,ncov s1,ncov s2,ncov spike,novel coronavirus RBD,novel coronavirus s1,novel coronavirus s2,novel coronavirus spike,RBD,S1,s2,Spike RBD|
|Immunogen||Recombinant 2019-nCoV S-trimer Protein (His Tag)(PKSR030489)|
|Recommended dilution||ELISA: 1:1,000-1:2,000|
|Storage instructions||Store at -20℃. Avoid freeze / thaw cycles.|
|Storage buffer||PBS, pH 7.4|
|Background||Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.|
Immobilized Recombinant 2019-nCoV S-trimer Protein (C-6His) (Cat#PKSR030489) at 5.0 ug/mL (100 uL/well) can bind Anti-2019-nCoV S-IgM Neutralizing Antibody (8A5), the EC50 is 10.1 ng/mL.
Immobilized Recombinant 2019-nCoV S Protein RBD-SD1 (C-6His) (Cat#PKSR030477) at 5.0 ug/mL(100 uL/well) can bind Anti-2019-nCoV S-IgM Neutralizing Antibody (8A5), the EC50 is 18.5 ng/mL.
Anti-2019-nCoV S-IgM Neutralizing Antibody(8A5) can block Human ACE-2 Protein (Avi-His Tag)(Cat#PKSR030493) and 2019-nCoV S-trimer Protein (Cat#PKSR030489) interaction, the IC50 for this effect is 83.9 ng/mL.