Alternate Names	PAS2,MNS,HGpMiX,GYPA,GPSAT
Swissprot	P10747
References	1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. June CH, et al. 1994. Immunol. Today 15:321. 3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.

Product Details

Form	Liquid
Clone No.	CD28.2(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Human CD28 Antibody[CD28.2]	50μg, 500μg, 1mg	Check More
Purified Anti-Human CD28 Antibody[CD28.2]	25μg, 100μg	Check More
FITC Anti-Human CD28 Antibody[CD28.2]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD28 Antibody[CD28.2]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD28 Antibody[CD28.2]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD28 Antibody[CD28.2]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD28 Antibody[CD28.2]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD28 Antibody[CD28.2]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD28 Antibody[CD28.2]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD28 Antibody[CD28.2]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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