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Cat.No.:E-AB-F1182UE
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Alternate Names | Ly94,mAR-1,NK-p46,mNKp46,Lymphocyte antigen 94 |
Swissprot | Q8C567 |
References |
1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ) 3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) |
Form | Liquid |
Clone No. | 29A1.4(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | APC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833E] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | APC |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |