Alternate Names	T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain,CD3E/D/G/Z,CD3e/d/g/z,CD3E/D/G/Z,CD3
Swissprot	P04235,P11942,P22646,P24161
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.

Product Details

Form	Liquid
Clone No.	17A2(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	APC Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843E]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD3 Antibody[17A2]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
Biotin Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
FITC Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD3 Antibody[17A2]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
PE Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
PerCP Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD3 Antibody[17A2]	25μg, 100μg	Check More

Citations

ACS Applied Materials & Interfaces (2022) IF: 10.383
Self-Activated Cascade Biocatalysis of Glucose Oxidase–Polycation–Iron Nanoconjugates Augments Cancer Immunotherapy

DOI: 10.1021/acsami.2c04894

Sample: Spleens
Marine Drugs (2022) IF: 5.118
In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

DOI: 10.3390/md20030197

PMID: 35323496
Sample: Dendritic cells (DCs)
JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
Immunomodulatory effects and mechanisms of Tiepishihu Xiyangshen granules on cyclophosphamide induced immuno-suppression via TLR4/MAPKs and PI3K/AKT/FOXO3a signal pathways

DOI: 10.1016/j.jep.2023.116192

Sample: T lymphocytes
Food & Function (2021) IF: 5.396
The petroleum ether extract of Brassica rapa L. induces apoptosis of lung adenocarcinoma cells via the mitochondria-dependent pathway

DOI: 10.1039/D1FO01547H

PMID: 34523644
Sample: spleen
Scientific Reports (2022) IF: 4.996
Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice

DOI: 10.1038/s41598-022-19031-9

PMID: 36056051
Sample: Spleen tissue
VACCINE (2021) IF: 3.641
Induction of Th1 and Th2 in the protection against SARS-CoV-2 through mucosal delivery of an adenovirus vaccine expressing an engineered spike protein

DOI: 10.1016/j.vaccine.2021.12.024

PMID: 34952759
Sample: splenocyte

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