Alternate Names	Receptor-type tyrosine-protein phosphatase C,Ptprc,L-CA,Ly-5,T200,CD45
Swissprot	P06800
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Product Details

Form	Liquid
Clone No.	30-F11(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	APC Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842E]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
PE Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
APC Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
PerCP Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More

Citations

INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
Dioscin modulates macrophages polarization and MDSCs differentiation to inhibit tumorigenesis of colitis-associated colorectal cancer

DOI: 10.1016/j.intimp.2023.109839

Sample: blood,spleen,myeloid-derived suppressor cells (MDS
Cell Death & Disease (2019) IF: 5.959
CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells

DOI: 10.1038/s41419-019-1796-6

PMID: 31332163
Sample: Cell culture supernatant
PeerJ (2022) IF: 2.984
3,4-dihydroxyphenylethyl alcohol glycoside reduces acetaminophen-induced acute liver failure in mice by inhibiting hepatocyte ferroptosis and pyroptosis

DOI: 10.7717/peerj.13082

PMID: 35310165
Sample: Liver tissue

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