Form	Liquid
Clone No.	RMV-7(See Other Available Formats)
Host	Rat
Isotype	Rat IgG1, κ
Isotype Control	APC Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823E]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD51 is a 140 kD protein, also known as αV integrin, vitronectin receptor, and integrin αV. It is a member of the integrin family, expressed on activated T cells, polymorphonuclear granulocytes, platelets, blastocysts, and osteoclasts. CD51 forms heterodimers by association with integrins β1, β3, β5 or β6; these complexes then act as receptors for multiple extracellular matrix proteins (ECM). The αvintegrin heterodimers have varied functions in development, stimulation/activation and homeostasis. The primary ligands for CD51 complexes are fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31. The RMV-7 antibody has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Biotin Anti-Mouse CD51 Antibody[RMV-7]	25μg, 100μg	Check More
PE Anti-Mouse CD51 Antibody[RMV-7]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD51 Antibody[RMV-7]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD51 Antibody[RMV-7]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD51 Antibody[RMV-7]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD51 Antibody[RMV-7]	25μg, 100μg	Check More

Citations

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