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APC Anti-Mouse CD73 Antibody[TY/23]

Uniprot : Q61503
  • Cat.No.:E-AB-F1089UE

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1089UE

Size:
  • 25μg
  • 100μg
Price: $72
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Antigen Details

Alternate Names 5'-nucleotidase,Nt5e,5'-NT,Ecto-5'-nucleotidase,CD73,Nt5, Nte,Nt5e
Swissprot Q61503
Gene ID 23959
References
1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.

Product Details

Form Liquid
Clone No. TY/23
Host Rat
Isotype Rat IgG2a, κ
Isotype Control APC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833E]
Reactivity Mouse
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD73 (ecto-5`-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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