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APC Anti-Mouse CD8a Antibody[53-6.7]

  • Cat.No.:E-AB-F1104UE

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1104UE

Size:
  • 25μg
  • 100μg
Price: $30
Qty:

Antigen Details

Alternate Names T-cell surface glycoprotein CD8 alpha chain,CD8A,T-lymphocyte differentiation antigen T8/Leu-2,MAL
Swissprot P01731
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Product Details

Form Liquid
Clone No. 53-6.7(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control APC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833E]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD8a Antibody[53-6.7] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
Biotin Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
FITC Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
PerCP Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse CD8a Antibody[53-6.7] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
PE Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
PerCP Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
PE/Elab Fluor® 594 Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse CD8a Antibody[53-6.7] 25μg, 100μg Check More

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Food & Function (2021) IF: 5.396
    The petroleum ether extract of Brassica rapa L. induces apoptosis of lung adenocarcinoma cells via the mitochondria-dependent pathway

    DOI: 10.1039/D1FO01547H

    PMID: 34523644

    Sample: spleen

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