Ascorbate Peroxidase (APX) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K353-S

      • 100Assays
      • 50Assays
      - +
      Price: $320
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(290 nm)

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      Detection principle

      Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.

      Performance characteristics

      Synonyms APX
      Sample type Plant tissue
      Sensitivity 0.071 U/g tissue
      Detection range 0.071-47 U/g tissue
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 60 min
      Precision Average inter-assay CV: 6.4%Average intra-assay CV: 4.8%
      Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months


      1. Melatonin Induction and Its Role in High Light Stress Tolerance in Arabidopsis thaliana
        IF: 15.221
        Journal: Journal of Pineal Research(2018)
        DOI: 10.1111/jpi.12504
        PMID: 29770489
        Products: E-BC-K353-S 
        Sample type: Leaf tissue
        Reactivity: Arabidopsis
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      MSDS for ELISA ISO 9001

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