Ascorbate Peroxidase (APX) Activity Assay Kit (E-BC-K353-S)
For research use only.
| Detection Principle | Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290. |
| Synonyms | APX |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (290 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.071 U/g tissue |
| Detection Range | 0.071-47 U/g tissue |
| Precision | inter-assay CV: 6.4% | intra-assay CV: 4.8% |
| Sample Volume | 100 μL(tissue homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Epipremnum aureum tissue homogenization | 1 |
| 10% Carrot tissue homogenization | 1 |
| 10% Green pepper tissue homogenization | 1 |
| 10% Mushrooms tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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