Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (Reitman-Frankel Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K236-M

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $180
      Can detect 43 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(500-520 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

      Performance characteristics

      Synonyms AST,GOT,Glutamic-oxalacetic transaminase
      Sample type Serum,plasma,animal tissue,cells,cell culture supernatant
      Sensitivity 1.1 IU/L
      Detection range 1.1-72.3 IU/L
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 80 min
      Precision Average inter-assay CV: 6.8%Average intra-assay CV: 5.3%
      Other instruments required Micropipettor, Vortex mixer, Incubator, Multichannel pipette
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Citations

      1. Noninvasive Visualization of Obesity-Boosted Inflammation in Orthotopic Pancreatic Ductal Adenocarcinoma Using an Octapod Iron Oxide Nanoparticle
        Journal: ACS Applied Bio Materials(2020)
        DOI: 10.1021/acsabm.0c00841
        PMID: 99999999
        Products: E-BC-K091-S E-BC-K235-M E-BC-K236-M 
        Sample type: Serum
        Reactivity: Mouse
      2. Hepatoprotective effect of Eugenol on Acetaminophen-Induced Hepatotoxicity in HepG2 cells
        Journal: Journal of Physics: Conference Series(2019)
        DOI: 10.1088/1742-6596/1374/1/012009
        PMID: 99999999
        Products: E-BC-K045 E-BC-K235-M E-BC-K236-M 
        Sample type: Cell sample
        Reactivity: Human
      3. Omega-3 fatty acids moderate oxidative and proinflammatory events in experimental hepatotoxicity in Wistar rats: comparison with livolin
        Journal: Synergy(2018)
        DOI: 10.1016/j.synres.2018.08.001
        PMID: 99999999
        Products: E-BC-K025-M E-BC-K031-M E-BC-K091-M E-BC-K096-M E-BC-K210 E-BC-K235-M E-BC-K236-M E-EL-0076 
        Sample type: Plasma
        Reactivity: Rat
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      Verified Customer

      a*****hSubmitted [ Jun 05 2019 ]

      • Application:Biochemical
      • Description:the biggest advantage of this kit is the small sample size.on the other hand,no special drawing tools are required,we can draw a standard curve in excel,which is convenient.
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      MSDS for ELISA ISO 9001

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