β-glucosidase (β-GC), also known as β-glucoside hydrolase, can efficiently hydrolyze the terminal non-reducing β-D-glucoside bonds in various glycoside compounds, thereby releasing β-D-glucose and corresponding ligands. The enzyme is widely present in nature, including the seeds of various plants, the small intestine and other organs of animals, and microorganisms.
β-GC is a very important industrial enzyme with various applications. In the field of medicine, β-GC catalyzes the degradation of glycoside groups to inhibit the expansion of cancer cells. In terms of diet, β-GC catalyzes the production of anti-cancer molecules isoflavones in soybean. In the energy field, β-GC is one of the key enzymes in the conversion of cellulose to glucose.
β-GC catalyzed the reaction of substrate to produce chromogenic substances at the wavelength of 400 nm, the maximum absorption of β-GC enzyme activity was reflected by measuring the OD value at 400 nm.
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