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β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

Cat.No.:E-BC-K785-M
Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

Detection method: Colorimetric method

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48T

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Detection principle

β- hydroxybutyrate dehydrogenase can catalyze the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm.

Performance characteristics

Synonyms	β-HB
Sample type	serum, plasma, urine, animal tissue
Sensitivity	0.01 mmol/L
Detection range	0.01-2.00 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 4%Average intra-assay CV: 2%
Other instruments required	Incubator, 50 kD Ultrafiltration tube, Microplate reader
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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