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β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

  • Cat.No.:E-BC-K785-M

  • Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

To Purchase E-BC-K785-M

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Price: $300

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Detection principle

β- hydroxybutyrate dehydrogenase can catalyze the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm.

Performance characteristics

Synonyms β-HB
Sample type serum, plasma, urine, animal tissue
Sensitivity 0.01 mmol/L
Detection range 0.01-2.00 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 60 min
Precision Average inter-assay CV: 4%Average intra-assay CV: 2%
Other instruments required Incubator, 50 kD Ultrafiltration tube, Microplate reader
Storage 2-8℃
Valid period 12 months


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