β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit (E-BC-F076)
For research use only.
| Detection Principle |
β-Secretase 1 is an aspartic protease, the full name is β-site amyloid precursor protein cleavage enzyme 1(BACE 1). BACE 1 cleaves amyloid precursor protein (APP) to produce amyloid-β peptide (Aβ) of 40 or 42 amino acids. The deposition of Aβ in plaques and vessel walls in the brains of patients with Alzheimer's disease is widely believed to be the major contributor to the disease. The detection principle of this kit is fluorescence resonance energy transfer (FRET) method. A fluorescence donor group and a quenching group are divided at both ends of the substrate. The fluorescence energy is transferred from the fluorescence donor to the quenching group, resulting in the attenuation of the fluorescence intensity of the fluorescence donor molecule itself. When the substrate is cleaved by BACE 1, the quenching group of the substrate and the fluorescent donor are separated and the fluorescent donor is no longer quenched, the fluorescence of the fluorescent donor can be detected, so that the enzymatic activity of BACE 1 can be calculated. The fluorescence produced by this kit had a maximum excitation wavelength of 325 nm and a maximum absorption wavelength of 393 nm. |
| Synonyms | BACE 1 |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=325 nm/393 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | DMSO |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.003 U/L |
| Detection Range | 0.003-4.75 U/L |
| Precision | inter-assay CV: 4.7-10.4% | intra-assay CV: 2.2-3.2% |
| Sample Volume | 10 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 2-4 |
| 10% Mouse kidney tissue homogenate | 2-4 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| Mouse plasma | 1 |
| 1.4×10^6 Jurkat cells | 1 |
| 1.4×10^6 A549 cells | 1 |
| 1.6×10^6 293T cells | 1 |
| 1.4×10^6 CHO cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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