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Biotin Anti-Human CD47 Antibody[CC2C6D4] E-AB-F1060B

Uniprot : Q08722
  • Cat.No.:E-AB-F1060B

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1060B

  • 25μg
  • 100μg
Price: $60

Antigen Details

Alternate Names Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP
Swissprot Q08722
1. Anstee DJ, et al. 1995. In Leucocyte Typing V (Schlossman ed.) Oxford University Press Oxford pp233-234.
2. Brown E, et al. 1990. J. Cell Biol. 111:2785.
3. Gao AG, et al. 1996. J. Biol. Chem. 271:21.
4. Lindberg FP, et al. 1994. J. Biol. Chem. 269:1567.

Product Details

Form Liquid
Clone No. CC2C6D4(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control Biotin Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793B]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Biotin

How to select the appropriate detection channel through the spectrogram?


CD47 also known as Rh-associated protein, gp42, integrin-associated protein (IAP), and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. Two splice variants have been described in the cytoplasmic tail, the shorter form is expressed in bone-marrow-derived cells, endothelial cells, and fibroblasts while the longer form is expressed by neural tissues. CD47 expression is widely distributed in hematopoietic cells including thymocytes, T cells, B cells, monocytes, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues. CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47; in the absence of CD47 mice show defects in host defense and β3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is also part of the Rh complex on erythrocytes.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry

Troubleshooting for Flow Cytometry


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