Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Biotin Anti-Human CD74 Antibody[LN2] E-AB-F1072B
Cat.No.:E-AB-F1072B
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Alternate Names | HLA class II histocompatibility antigen gamma chain,CD74,Ia antigen-associated invariant chain,Ii,p33,DHLAG |
Swissprot | P04233 |
References |
1. Moldenhauer G, et al. 1999. Immunology 96:473.
2. Shi X, et al. 2006. Immunity 25:595. 3. Beswick EJ, et al. 2006. Infect. Immun. 74:1148. 4. Zola H,et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication. |
Form | Liquid |
Clone No. | LN2(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | Biotin Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793B] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Biotin |
How to select the appropriate detection channel through the spectrogram?
CD74 is a type II transmembrane glycoprotein also known as MHC class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, and MIF receptor. CD74 exists in four isoforms with molecular masses of 33, 35, 41, and 43 kD, depending on genetic splicing. CD74 is primarily expressed on antigen presenting cells, including B cells, monocytes/macrophages, dendritic cells, and Langerhans cells. It is also expressed by activated T cells and activated endothelial and epithelial cells as well as carcinomas of lung, renal, gastric and thymic origin. The primary function of CD74 is intracellular sorting of MHC class II molecules and regulation of exogenous peptide loading onto MHC class II. It is also involved in the modulation of B cell differentiation and positive selection of CD4+ T cells. It has been reported that CD74 binds MIF (macrophage migration inhibitory factor) and signals through CD44 to regulate innate and adaptive immunity. It is also reported that H. pylori infection occurs through urease B binding of CD74 on gastric epithelial cells, inducing gastric epithelial cell apoptosis, NF-κB activation, and IL-8 production. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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