Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Biotin Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034B
Cat.No.:E-AB-F1034B
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Alternate Names | P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162 |
Swissprot | Q62170 |
References |
1. Borges E, Eytner R, Moll T, et al.Blood. 1997; 90(5):1934-1942.
2. Borges E, Tietz W, Steegmaier M, et al. J Exp Med. 1997; 185(3):573-578. 3. Frenette PS, Denis CV, Weiss L, et al. . J Exp Med. 2000; 191(8):1413-1422. |
Form | Liquid |
Clone No. | 4RA10(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG1, κ |
Isotype Control | Biotin Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823B] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Biotin |
How to select the appropriate detection channel through the spectrogram?
The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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