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Biotin Quantitative Determination Kit

  • Cat.No.:E-IR-R501

  • Application: ELISA

To Purchase E-IR-R501

Size:
  • 96T
  • 96T*5
  • 96T*10
Price: $320
Qty:

Introduction

Intended Use: This ELISA kit applies to the in vitro quantitative determination of Biotin concentrations in biological fluids.

Detection Range: 0.31–20 ng/mL
Sensitivity: 0.19 ng/mL
Specificity: This kit recognizes Biotin. No significant cross-reactivity or interference was observed
Repeatability: Coefficient of variation is <10%

Method for use

1. Add 50μL standard or sample to each well.
2. Immediately add 50 μL Avidin-HRP working solution to each well.
3. Incubate for 30 min at 37℃.
4. Aspirate and wash 5 times.
5. Add 90 μL Substrate Reagent. Incubate 15 min at 37℃.
6. Add 50 μL Stop Solution. Read at 450 nm immediately.
7. Calculation of results.

Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the manual since the kit is received and it is valid for 12 months.

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. ACS Applied Materials & Interfaces (2022) IF: 9.2289
    Multifunctional Self-Assembly with NIR Light-Activated Cascade Effect for Improving Local Treatment on Solid Tumors

    DOI: 10.1021/acsami.2c00448

    PMID: 35297244

  2. PLoS Pathogens (2023) IF: 7.464
    The opportunistic pathogen Pseudomonas aeruginosa exploits bacterial biotin synthesis pathway to benefit its infectivity

    DOI: 10.1371/journal.ppat.1011110

    Sample: plasma,heart,liver,spleen,lungs,kidneys,large inte
  3. METABOLIC ENGINEERING (2019) IF: 7.808
    Multi-level metabolic engineering of Pseudomonas mutabilis ATCC31014 for efficient production of biotin

    DOI: 10.1016/j.ymben.2019.05.005

  4. AQUACULTURE (2021) IF: 4.242
    Interactive effects of dietary biotin and carbohydrate on growth performance and glucose metabolism in juvenile turbot Scophthalmus maximus L.

    DOI: 10.1016/j.aquaculture.2021.736752

    Sample: Whole Blood,Tissue Slice
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