Current Position:Home>>Kits>>Metabolism Assays>> Blood Ammonia Colorimetric Assay Kit

Blood Ammonia Colorimetric Assay Kit

Cat.No.:E-BC-K145-M
Detection instrument: Microplate reader(600-660 nm,optimum wavelength: 635 nm)

Detection method: Colorimetric method

To Purchase E-BC-K145-M

Size:

96T
48T

Price: $260

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Blood protein can be precipitated with protein precipitator, and enzyme activity will be destroyed, which can prevent the formation of free ammonia in vitro. Most interfering color substances were removed at the same time, indigo was formed in non-protein filtrate by Berthelot reaction, and the color depth was proportional to the content of blood ammonia. Blood ammonia content can be determined by comparing with standard solution.

Performance characteristics

Synonyms	NH3
Sample type	Serum,plasma
Sensitivity	0.01 mmol/L
Detection range	0.01-2.5 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	45 min
Precision	Average inter-assay CV: 7.200%Average intra-assay CV: 4.100%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-2.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Mouse serum	1
Rat plasma	1
Dog serum	1
Horse serum	1

Note: The diluent is double distilled water or normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

NEUROSCIENCE LETTERS (2021) IF: 3.046
Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

DOI: 10.1016/j.neulet.2021.136385

PMID: 34871743
Sample: serum
Frontiers in Medicine (2020) IF: 3.9
Helicobacter pylori as an Initiating Factor of Complications in Patients With Cirrhosis: A Single-Center Observational Study

DOI: 10.3389/fmed.2020.00096

Sample: serum

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

Cat. No	E-BC-K145-M
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha