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Blood Ammonia Colorimetric Assay Kit

  • Cat.No.:E-BC-K145-M

  • Detection instrument: Microplate reader(600-660 nm,optimum wavelength: 635 nm)

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Price: $260

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Detection principle

Blood protein can be precipitated with protein precipitator, and enzyme activity will be destroyed, which can prevent the formation of free ammonia in vitro. Most interfering color substances were removed at the same time, indigo was formed in non-protein filtrate by Berthelot reaction, and the color depth was proportional to the content of blood ammonia. Blood ammonia content can be determined by comparing with standard solution.

Performance characteristics

Synonyms NH3
Sample type Serum,plasma
Sensitivity 0.01 mmol/L
Detection range 0.01-2.5 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 45 min
Precision Average inter-assay CV: 7.2%Average intra-assay CV: 4.1%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 3 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-2.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Human plasma


Mouse serum


Rat plasma


Dog serum


Horse serum


Note: The diluent is double distilled water or normal saline (0.9% NaCl).


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