BRSK1 Polyclonal Antibody
- +4
Price: $ 530
Price: $ 320
Price: $ 200
Size:
200μL
120μL
60μL
Quantity:
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: IHC
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Product Details
Verified Samples |
Verified Samples in IHC:Rat liver,Rat brain,Human stomach,Mouse liver,Mouse testis,Mouse brain |
Dilution |
IHC 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant fusion protein of human BRSK1 (NP_115806.1). |
Abbre | BRSK1 |
Synonyms | BRSK1;hSAD1 |
Swissprot | |
Cellular Localization | Cytoplasm. Nucleus. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Cell Biology |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. BRSK1 (BR serine/threonine-protein kinase 1), also known as SAD1, is a 794 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one UBA domain and one protein kinase domain. Expressed in a variety of tissues with highest expression in testis and brain, BRSK1 uses magensium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins, including Wee 1 and Cdc25B. Via its kinase activity toward proteins that are involved in microtubule assembly, BRSK1 plays an essential role in neuronal polarization and may be involved in regulating cell cycle arrest in response to DNA damage, Two isoforms of BRSK1 exist due to alternative splicing events. |
Experiment Operation Video
Protocols
• Western Blot Guide
• Immunohistochemistry Guide for Slide-mounted Paraffin Sections
• Immunofluorescence Guide
• Immunohistochemistry Troubleshooting Tips
• Western Blot Troubleshooting Tips
• Immunofluorescence Troubleshooting Tips
• Comparison of Protein Separation Linear Range
• ImmunoPrecipitation Protocol