English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

c-Fos Monoclonal Antibody

Cat:E-AB-70204 Citations (1)
Manual MSDS
  • +2

Price: $ 410

Price: $ 260

Price: $ 160

Size:
200μL 120μL 60μL
Quantity:
  • Host: Mouse
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC;IF
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:HepG2,Jurkat,Human colon cancer,Mouse lung
Verified Samples in IHC:Human gallbladder,Mouse brain
Verified Samples in IF:Rat brain
Dilution

WB 1:500-1:2000, IHC 1:500-1:2000, IF 1:200-1:1000

Western Blot Operation Guide
Clonality Monoclonal
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse c-FOS
Abbre c-Fos
Synonyms Activator protein 1;AP 1;C FOS;Cellular oncogene c fos;Cellular oncogene fos;FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS);FBJ murine osteosarcoma viral oncogene homolog;FBJ murine osteosarcoma viral v fos oncogene homolog;FBJ Osteosarcoma Virus;FOS;FOS protein;FOS;G0 G1 switch regulatory protein 7;G0/G1 switch regulatory protein 7;G0S7;Oncogene FOS;p55;proto oncogene c Fos;Proto oncogene protein c fos;Proto-oncogene c-Fos;v fos FBJ murine osteosarcoma viral oncogene homolog
Swissprot
Calculated MW 62kDa
Observed MW 62kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 0.97 mg/mL
Buffer PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Epigenetics and Nuclear Signaling; Neuroscience
Clone No. 4B4F11
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.