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Ca2+-ATPase Activity Assay Kit

Cat.No.:E-BC-K212-S
Detection instrument: Spectrophotometer (660nm)

Detection method: Colorimetric method

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Detection principle

ATPase exists on the membrane of tissue cells and organelles. It is a kind of protease on the biological membrane which plays an important role in material transport, energy conversion and information transmission. Ca2+ which participates in the regulation of different enzyme systems and cell activities plays many important roles in cells. The flow of Ca2+ depends on the Ca2+ -ATPase on the cell membrane, and Ca2+ -ATPase consumes ATP to generate the energy needed for ion transport.

Performance characteristics

Synonyms	Ca2+-ATPase
Sample type	Animal tissue and cell
Sensitivity	0.8 U/g wet weight
Detection range	0.8-41 U/g wet weight
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	50 min
Precision	Average inter-assay CV: 8.800%Average intra-assay CV: 4.100%
Other instruments required	Test tube, Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

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Collection and treatment of common samples for metabolism assay detection

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