Na+k+-ATPase Activity Assay Kit (E-BC-K539-M)
                        For research use only.
| Detection Principle | Na+K+ -ATPase decomposes ATP to produce ADP and Phosphorus, and calculates the activity of Na+K+ -ATPase by measuring the content of phosphorus. | 
| Synonyms | Na+K+-ATPase | 
| Sample Type | Serum,Plasma,Animal tissue | 
| Detection Method | Colorimetric method | 
| Detection Instrument | Microplate reader (640-680 nm, optimum wavelength: 660 nm) | 
| Research Area | Oxidative Stress | 
| Other Reagents Required | Normal saline (0.9% NaCl) | 
| Storage | This product can be stored at 2-8°C for 12 months with shading light. | 
| Valid Period | 12 months | 
| Sensitivity | 0.11 μmol Pi/mL/hour | 
| Detection Range | 0.42-4.99 μmol Pi/mL | 
| Precision | inter-assay CV: 2.2% | intra-assay CV: 2.2% | 
| Sample Volume | 200 μL | 
| Assay Time | 35 min | 
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor | 
|---|---|
| Rat plasma | 1 | 
| 10% Rat liver tissue homogenate | 6-10 | 
| 10% Rat spleen tissue homogenate | 4-8 | 
| 10% Rat heart tissue homogenate | 4-8 | 
| 10% Mouse brain tissue homogenate | 4-8 | 
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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Biotin
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Cyanine 3
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Elab Fluor® 430
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