English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

Calcium (Ca) Colorimetric Assay Kit

Cat:E-BC-K103-M Citations (13)
Manual MSDS

Price: $ 150

Price: $ 120

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;urine;tissue
  • Detection Instrument: Microplate reader(600-620 nm,optimum wavelength: 610 nm)
  • Reasearch Areas: Inorganic ions;Plant Physiology Series
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Detection Principle Calcium ion in the sample bind to Methyl Thymol Blue (MTB) in alkaline solution and form blue complex. The blue complex has a specific absorption peak at 715nm and calcium content can be calculated by measuring the OD value at 610 nm.
Synonyms Ca
Sample Type serum,plasma,urine,tissue
Sensitivity 0.07 mmol/L
Detection Range 0.07-1.2 mmol/L
Detection Method Colorimetric method
Assay Time 20 min
Precision inter-assay CV: 8.5% | intra-assay CV: 4.7%
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Dog serum 2-3
Human serum 3-6
Mouse serum 3-6
Human urine 4-8
20% Animal tissue homogenate 1
The diluent is deionized water. For the dilution of other sample types, please do pretest to confirm the dilution factor.