Potassium (K) turbidimetric Assay Kit (E-BC-K279-M)
For research use only.
| Detection Principle | Under the alkaline condition, the sodium tetraphenylborate reacts with the potassium ions in the sample to form the potassium tetraphenylborate which is white and small particles with small solubility. Potassium tetraphenylborate particles are in a stable suspension state in the solution. The turbidity is proportional to the potassium ion concentration in the sample and potassium content can be calculated indirectly by measuring the OD value at 450 nm. |
| Synonyms | K |
| Sample Type | Serum,Plasma,Milk,Animal tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450-600 nm, optimum wavelength: 450 nm) |
| Research Area | Inorganic Ions , Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.002 mmol/L |
| Detection Range | 0.01-0.80 mmol/L |
| Precision | inter-assay CV: 6.1% | intra-assay CV: 1.1% |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| RAW 264.7 cellular supernatant | 1 |
| Human plasma | 1 |
| Human milk | 1 |
| 10% Rat liver tissue homogenization | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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