Catalase (CAT) Activity Assay Kit (E-BC-K031-M)
For research use only.
| Detection Principle | The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm. |
| Synonyms | CAT |
| Sample Type | Serum,Plasma,Cell,Cell culture supernatant,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-410 nm, optimum wavelength: 405 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.12 U/mL |
| Detection Range | 1.12-150 U/mL |
| Precision | inter-assay CV: 7.7% | intra-assay CV: 3.9% |
| Sample Volume | 40 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| 293T supernatant | 1 |
| 10% Rat heart tissue homogenization | 50-100 |
| 10% Rat liver tissue homogenization | 100-200 |
| 10% Rat spleen tissue homogenization | 50-100 |
| Mouse serum | 1 |
| 10% Epipremnum aureum tissue homogenization | 1-2 |
| 10% Rat lung tissue homogenization | 50-100 |
| 10% Rat kidney tissue homogenization | 50-100 |
| 10% Rat brain tissue homogenization | 20-50 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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