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Catalase (CAT) Activity Fluorometric Assay Kit

Cat.No.:E-BC-F006
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

Detection method: Fluorescence method

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96T

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Protocols for metabolism assays

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Detection principle

Catalase can decompose H2O2 to generate H2O and O2, the residual H2O2 in the detection system react with the fluorescent substance, and the content of residual H2O2 is proportional to the fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm, the catalase activity is inversely proportional to the fluorescence intensity.

Performance characteristics

Synonyms	CAT
Sample type	Serum, plasma, animal tissue
Sensitivity	0.01 U/L
Detection range	0.01-6.51 U/L
Detection method	Fluorescence method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 6.800%Average intra-assay CV: 3.400%
Other instruments required	Vortex mixer, Centrifuge
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-6.51 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Mouse serum	60-80
Mouse plasma	60-80
Rat serum	70-80
Human saliva	30-50
Rat urine	30-50
10% Mouse liver tissue homogenate	2500-3000
10% Mouse lung tissue homogenate	200-400
10% Rat muscle tissue homogenate	100-200
10% Rat brain tissue homogenate	40-50
10% Mouse kidney tissue homogenate	2000-2500

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

DOI: 10.1016/j.fct.2021.112124

PMID: 33727180
Sample: Tissue homogenate

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