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CDX2 Monoclonal Antibody

Cat:E-AB-22020
Manual MSDS
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Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p;IF
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Product Details
Verified Samples Verified Samples in WB:293T,Mouse heart
Verified Samples in IHC:Human colon
Verified Samples in IF:Mouse kidney
Dilution

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Synthetic Peptide
Abbre CDX2
Synonyms Caudal type homeo box 2;Caudal type homeo box transcription factor 2;Caudal type homeobox 2;Caudal type homeobox protein 2;Caudal type homeobox transcription factor 2;Caudal-type homeobox protein 2;CDX 2;CDX 3;CDX-3;Cdx2;CDX2/AS;CDX2;CDX3;Homeobox protein CDX 2;Homeobox protein CDX-2
Swissprot
Calculated MW 34kDa
Observed MW 42kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method Protein A purification
Research Areas Cancer; Epigenetics and Nuclear Signaling; Developmental Biology; Stem Cells
Clone No. Clone:1A5
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis.