Cell Mitochondrial Complex Ⅴ (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M)
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For research use only.
| Detection Principle | Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. The activity of mitochondrial complex V can be quantified by measure the change OD value at 340 nm. |
| Synonyms | ATP Synthase;F0F1-ATPase |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Research Area | Mitochondria Function , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 3.40 U/L |
| Detection Range | 3.40-272.07 U/L |
| Precision | inter-assay CV: 3.0-6.5% | intra-assay CV: 3.0-4.0% |
| Sample Volume | 20 μL(cell homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 Hela cells | 1 |
| 4×10^6 CHO cells | 1 |
| 1 × 10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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