Cell Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit (E-BC-K837-M)
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For research use only.
| Detection Principle | Mitochondrial complex IV, also known as cytochrome C oxidase, is one of the major enzymes in the mitochondrial respiratory chain. It oxidizes the reduced cytochrome C converted from mitochondrial complex III to oxidized cytochrome C and consumes oxygen to generate water. Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm. |
| Synonyms | Cytochrome C Oxidase |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (540-560 nm, optimum wavelength: 550 nm) |
| Research Area | Mitochondria Function , Energy Metabolism |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.79 U/L |
| Detection Range | 0.79-47.0 U/L |
| Precision | inter-assay CV: 4.0-9.5% | intra-assay CV: 3.0-5.5% |
| Sample Volume | 40 μL(cell homogenate) |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1×10^6 Jurkat cells | 1 |
| 4×10^6 CHO cells | 1 |
| 1×10^6 K562 cells | 1 |
| 1 × 10^6 HL-60 cells | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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