Current Position:Home>>Kits>>ELISA Kits>> cGMP(Cyclic GMP) ELISA Kit
Cat.No.:E-EL-0083
Welcome to order from local distributors.
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 0.78-50 pmol/mL |
Sensitivity | 0.47 pmol/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes cGMP in samples. No significant cross-reactivity or interference between cGMP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of cGMP concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level cGMP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level cGMP were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pmol/mL) | 98.40 | 299.90 | 999.00 | 92.40 | 326.30 | 1055.70 |
Standard deviation | 5.20 | 13.50 | 35.00 | 4.80 | 18.60 | 53.80 |
CV (%) | 5.28 | 4.50 | 3.50 | 5.19 | 5.70 | 5.10 |
The recovery of cGMP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 91-104 | 96 |
EDTA plasma(n=8) | 91-108 | 99 |
Cell culture media(n=8) | 86-97 | 91 |
Research Area | Signal transduction |
![]() |
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
![]() |
2. Aspirate and wash the plate for 3 times |
![]() |
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
![]() |
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
![]() |
5. Add 50μL Stop Solution |
![]() |
6. Read the plate at 450nm immediately. Calculation of the results |
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!
PMID: 35935981 |
Sample: MDSC |
PMID: 33186350 |
Sample: Cell sample |
PMID: 36115153 |
Sample: Penile |
PMID: 35247715 |
Sample: Penile |
DOI: 10.1111/cns.13807 |
|
Sample: spinal cord |
Sample: UMR106 cell |
PMID: 36034809 |
Sample: cardiomyocytes (CMs),cardiac fibroblasts (CFs) |
PMID: 36157749 |
Sample: serum |
PMID: 34229480 |
Sample: serum,testis |
Sample: Serum |
PMID: 34530094 |
Sample: serum |
DOI: 10.1530/REP-20-0381 |
|
PMID: 33112813 |
Sample: testis |
DOI: 10.1038/aps.2017.129 |
|
Sample: Tissue homogenate |
PMID: 35850398 |
Sample: hippocampal tissue |
PMID: 30315252 |
Sample: Tissue homogenate |
PMID: 36099318 |
Sample: penile |
PMID: 36105026 |
Sample: plasma |
PMID: 32295540 |
Sample: Tissue homogenate |
DOI: 10.1155/2018/2813025 |
|
Sample: plasma |
Sample: heart tissue,plasma |
DOI: 10.3390/cimb45020108 |
|
Sample: epididymal tissue |
Sample: pancreatic tissue,BxPC-3 cell,CAPAN2 cell |
DOI: 10.1111/and.13344 |
|
Sample: corpus cavernosum |
Sample: Placenta |
Sample: plasma |