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Chlorine (Cl) Colorimetric Assay Kit

Cat.No.:E-BC-K189-M
Detection instrument: Microplate reader(440 nm-480 nm,optimum wavelength: 460 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Chloride ion in biological fluids are replaced by the mercury ions in mercury thiocyanate through ion replacement, which resulted in the formation of difficult-to-dissociate mercury chloride. The substituted thiocyanate ions were combined with ferric nitrate to form a red complex. The content of chlorine ion can be calculated indirectly by measuring the OD value at 460 nm.

Performance characteristics

Synonyms	Cl
Sample type	Serum, plasma, animal tissue
Sensitivity	1 mmol/L
Detection range	1.0-60 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	15 min
Precision	Average inter-assay CV: 6.400%Average intra-assay CV: 3.600%
Other instruments required	Micropipettor, Tubes, Vortex mixer, Incubator, Centrifuge.
Storage	2-8℃
Valid period	12 months

Images

The chloride concentration in supernatant was significantly increased in HG group and reduced in IAA94 treated group. (**P<0.01 vs. CON, ##P<0.01 vs. HG)

SH Li et al investigate the diabetic cardiomyopathy induced by sleeve gastrectomy.. Chloride (Cl) concentration in cell supernatant was determined using chloride colorimetric assay kit (E-BC-K189-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.0-60 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Mouse liver tissue homogenate	2-3
10% Mouse kidney tissue homogenate	2-3
10% Rat spleen tissue homogenate	2-3
Human serum	5-10
Mouse serum	5-10
Cynomolgus monkey serum	5-10

Note: The diluent is double distilled water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy

DOI: 10.1155/2022/4608914

PMID: 35498125
Sample: H9c2 cell
ENVIRONMENTAL TECHNOLOGY (2023) IF: 3.475
Reuse of composted rural food waste in China as vermicomposting substrate: Effects on earthworms, associated microorganisms, and economic benefits

DOI: 10.1080/09593330.2023.2184728

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