Creatine kinase (CK) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K558-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $500
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(340 nm)

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      Detection principle

      Creatine kinase (CK) catalyze creatine phosphate and ADP to produce creatine and ATP. Hexokinase catalyze creatine and glucose to produce glucose-6-phosphate. Glucose-6-phosphate dehydrogenase (G-6-PD) catalyze glucose-6-phosphate and NADP+ to produce NADPH which have a maximum absorption peak at 340 nm. The CK activity can be calculated by measuring the OD values at 340 nm.

      Performance characteristics

      Synonyms CK,Creatine phosphokinase
      Sample type Serum,plasma,animal tissue,cells
      Sensitivity 3.7 U/L
      Detection range 3.7-160 U/L
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 80 min
      Precision Average inter-assay CV: 8.1%Average intra-assay CV: 5.2%
      Other instruments required Micropipettor, Incubator, Vortex mixer
      Other reagents required PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 3 months

      Related Products

      Citations

      1. Protective Effect of Barbaloin in a Rat Model of Myocardial Ischemia Reperfusion Injury Through the Regulation of the CNPY2-PERK Pathway
        IF: 2.928
        Journal: International Journal of Molecular Medicine(2019)
        DOI: 10.3892/ijmm.2019.4123
        PMID: 30864682
        Products: E-BC-K046-M E-BC-K558-S 
        Sample type: Serum
        Reactivity: Rat
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      MSDS for ELISA ISO 9001

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