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Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)

Cat.No.:E-BC-K188-M
Detection instrument: Microplate reader(510-520 nm,optimum wavelength: 515 nm)

Detection method: Colorimetric method

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Detection principle

Creatinine (Cr) can be catalyzed by creatinase and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm.

Performance characteristics

Synonyms	Cr
Sample type	Serum,plasma,urine
Sensitivity	3.8 μmol/L
Detection range	20.45-400 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 3.700%Average intra-assay CV: 1.400%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Images

No significant change of creatinine was observed, the LCFG nanoparticles did not include nephrotoxicity.

X Zhang et al investigate the usage of H2O2 and O2 for cooperative chemodynamic/starvation cancer therapy. Creatinine (Cr) level in mouse blood was determined using creatinine colorimetric assay kit (E-BC-K188-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (38.2-800 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human urine	20-30
Human serum	1
Rat serum	1
Porcine serum	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

BIOMATERIALS (2021) IF: 12.479
Cyclic reactions-mediated self-supply of H2O2 and O2 for cooperative chemodynamic/starvation cancer therapy

DOI: 10.1016/j.biomaterials.2021.120987

PMID: 34175561
Sample: MDA-MB-231 cells
Acta Biomaterialia (2021) IF: 8.9469
An efficient and safe MUC1-dendritic cell-derived exosome conjugate vaccine elicits potent cellular and humoral immunity and tumor inhibition in vivo

DOI: 10.1016/j.actbio.2021.10.041

PMID: 34757230
Sample: serum
Acta Biomaterialia (2021) IF: 8.9469
A solid lipid coated calcium peroxide nanocarrier enables combined cancer chemo/chemodynamic therapy with O2/H2O2 self-sufficiency

DOI: 10.1016/j.actbio.2020.12.036

Sample: Serum,Plasma
Biomaterials Science (2022) IF: 7.59
Transdermal delivery of allopurinol on acute hyperuricemic mice via polymer microneedles for regulation of serum uric acid levels

DOI: 10.1039/D2BM01836E

Sample: serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

DOI: 10.3390/ijms232315400

Sample: plasma,urine
PHYTOTHERAPY RESEARCH (2022) IF: 6.388
Resveratrol reverses TGF-β1-mediated invasion and metastasis of breast cancer cells via the SIRT3/AMPK/autophagy signal axis

DOI: 10.1002/ptr.7608

PMID: 36086852
Sample: serum
Pharmaceutics (2023) IF: 6.525
Ultrasonically Fabricated Beta-Carotene Nanoemulsion: Optimization, Characterization and Evaluation of Combinatorial Effect with Quercetin on Streptozotocin-Induced Diabetic Rat Model

DOI: 10.3390/pharmaceutics15020574

Sample: serum
Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
Capsaicin Prevents Contrast-Associated Acute Kidney Injury through Activation of Nrf2 in Mice

DOI: 10.1155/2022/1763922

PMID: 35615576
Sample: serum
CELL BIOLOGY AND TOXICOLOGY (2023) IF: 6.819
Syringaresinol protects against diabetic nephropathy by inhibiting pyroptosis via NRF2-mediated antioxidant pathway

DOI: 10.1007/s10565-023-09790-0

Sample: urine
JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
Yishen Tongluo formula alleviates diabetic kidney disease through regulating Sirt6/TGF-β1/Smad2/3 pathway and promoting degradation of TGF-β1

DOI: 10.1016/j.jep.2023.116243

Sample: kidney
Frontiers in Pharmacology (2022) IF: 5.811
Stewed Rhubarb Decoction Ameliorates Adenine-Induced Chronic Renal Failure in Mice by Regulating Gut Microbiota Dysbiosis.

DOI: 10.3389/fphar.2022.842720

Sample: plasma
Drug Design Development and Therapy (2021) IF: 4.162
Total Glucosides of Paeony Inhibited Autophagy and Improved Acute Kidney Injury Induced by Ischemia-Reperfusion via the lncRNA TUG1/miR-29a/PTEN Axis

DOI: 10.2147/DDDT.S286606

PMID: 34079224
Sample: Serum
Drug Design Development and Therapy (2021) IF: 4.162
Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

DOI: 10.2147/DDDT.S326975

PMID: 34703210
Sample: serum
Biomed Research International (2022) IF: 3.246
Evaluation Value of Serum miR-4299 and miR-16-5p in Risk Stratification of Sepsis-Induced Acute Kidney Injury

DOI: 10.1155/2022/5165892

PMID: 35845963
Sample: serum
Dose-Response (2022) IF: 2.623
Biochemical Investigation of Therapeutic Potentials of Plant-Based Bioactive Compounds as Stimulators of Glucagon like peptide-1 Secretion :

DOI: 10.1177/15593258221114184

Sample: serum
SMALL RUMINANT RESEARCH (2022) IF: 1.611
Meat production, feed and water efficiencies of selected South African sheep breeds

DOI: 10.1016/j.smallrumres.2022.106746

Sample: urine
DRUG DEVELOPMENT RESEARCH (2019) IF: 1.742
Montelukast modifies simvastatin‐induced myopathy and hepatotoxicity

DOI: 10.1002/ddr.21581

Sample: liver

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