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CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Cat.No.:E-BC-K022-M
Detection instrument: Microplate reader(530-570 nm,optimum wavelength: 550 nm)

Detection method: Colorimetric method

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Detection principle

Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

Performance characteristics

Synonyms	CuZn-SOD,Mn-SOD,T-SOD
Sample type	Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity	1.35 U/mL
Detection range	1.35-62 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 9.600%Average intra-assay CV: 5.100%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	Reagent 4: -20℃, others: 2-8℃
Valid period	12 months

Images

The activity of SOD was significantly changed between different superscripted letters. (P<0.05)

Y M Alharbi et al investigate the antioxidative and abtidiabetic effect of probiotic-enriched fermented camel milk combined with Salvia officinalis leaves extract. Superoxide dismutase (SOD) activity of rat blood was determined using SOD activity assay kit (E-BC-K022-M).

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Mouse serum	5-10
Rat serum	6-15
Urine	2-3
Human hydrothorax	2-3
10% Mouse liver tissue homogenate	100-200
10% Mouse brain tissue homogenate	20-30
10% Mouse kidney tissue homogenate	50-120
10% Rat kidney tissue homogenate	50-120
HepG2 cells (5.21mgprot/mL)	15-25
Cell culture supernatant	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

PLANT CELL AND ENVIRONMENT (2022) IF: 7.228
The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

DOI: 10.1111/pce.14263

PMID: 35040151
Sample: leaf
PLANT CELL AND ENVIRONMENT (2022) IF: 7.228
The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

DOI: 10.1111/pce.14263

PMID: 35040151
Sample: leaf
ARCHIVES OF TOXICOLOGY (2023) IF: 6.168
The circadian rhythm gene Bmal1 ameliorates acute deoxynivalenol-induced liver damage

DOI: 10.1007/s00204-022-03431-x

Sample: liver,serum
Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation

DOI: 10.1155/2022/7655142

Sample: nucleus pulposus(NP) cell
Nutrients (2022) IF: 6.706
Ascophyllum nodosum and Fucus vesiculosus Extracts Improved Lipid Metabolism and Inflammation in High-Energy Diet–Induced Hyperlipidemia Rats

DOI: 10.3390/nu14214665

Sample: serum
LIFE SCIENCES (2022) IF: 5.037
sSTEAP4 regulates cellular homeostasis and improves high-fat-diet-caused oxidative stress in hepatocytes

DOI: 10.1016/j.lfs.2022.120438

PMID: 35227772
Sample: serum
Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

DOI: 10.1155/2020/8365056

PMID: 32724495
Sample: Tissue homogenate
Foods (2022) IF: 5.561
Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

DOI: 10.3390/foods11193048

PMID: 36230124
Sample: serum
Journal of Environmental Sciences (2022) IF: 5.565
Alleviative effects of a novel strain Bacillus coagulans XY2 on copper-induced toxicity in zebrafish larvae

DOI: 10.1016/j.jes.2022.02.010

PMID: 36375957
Sample: larvae
LIVER TRANSPLANTATION (2022) IF: 5.799
Cerium oxide nanoparticles administration during machine perfusion of discarded human livers: a pilot study

DOI: 10.1002/lt.26421

PMID: 35100468
Sample: liver
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Separation and Identification of Resveratrol Butyrate Ester Complexes and Their Bioactivity in HepG2 Cell Models

DOI: 10.3390/ijms222413539

PMID: 34948341
Sample: HepG2 cell
Frontiers in Pharmacology (2022) IF: 5.988
Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.

DOI: 10.3389/fphar.2022.961011

Sample: testicular tissue
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cells

DOI: 10.1016/j.jep.2022.115110

PMID: 35181488
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Amelioration of risk factors associated with diabetic nephropathy in diet-induced pre-diabetic rats by an uracil-derived diimine ruthenium(II) compound

DOI: 10.1016/j.biopha.2020.110483

PMID: 32768965
Sample: Plasma,Tissue homogenate
CANCER SCIENCE (2020) IF: 4.966
DRP1 promotes lactate utilization in KRAS‐mutant non‐small‐cell lung cancer cells

DOI: 10.1111/cas.14603

Sample: Cell culture supernatant
Bioengineered (2022) IF: 3.269
Methylprednisolone up-regulates annexin A1 (ANXA1) to inhibit the inflammation, apoptosis and oxidative stress of cigarette smoke extract (CSE)-induced bronchial epithelial cells, a chronic obstructive pulmonary disease in vitro model, through the formyl

DOI: 10.1080/21655979.2022.2031769

Sample: BEAS-2B cells
Brain and Behavior (2022) IF: 3.405
The protective effects of nesfatin-1 in neurological dysfunction after spinal cord injury by inhibiting neuroinflammation

DOI: 10.1002/brb3.2778

PMID: 36271663
Sample: Spinal cord injury (SCI) area
BRAIN RESEARCH BULLETIN (2022) IF: 3.715
Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke

DOI: 10.1016/j.brainresbull.2022.09.015

PMID: 36152772
Sample: brain tissue
Annals of Translational Medicine (2022) IF: 3.932
Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

DOI: 10.21037/atm-22-483

PMID: 35280364
Sample: myocardium
Scientific Reports (2020) IF: 3.998
Cyclovirobuxine D protects against diabetic cardiomyopathy by activating Nrf2-mediated antioxidant responses

DOI: 10.1038/s41598-020-63498-3

Sample: cardiac tissue
CRYOBIOLOGY (2020) IF: 2.287
Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen

DOI: 10.1016/j.cryobiol.2020.04.010

PMID: 32437678
Sample: Sperm
BIOLOGICAL RHYTHM RESEARCH (2022) IF: 1.219
Impact of photoperiods on the specific activities of immune and antioxidant enzymes in different tissues of Dybowski’s frog (Rana dybowskii)

DOI: 10.1080/09291016.2022.2043588

Sample: serum,liver,skin
Veterinary World (2020)
Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

DOI: 10.14202/vetworld.2020.1404-1409

PMID: 32848317
Sample: Serum

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