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CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Cat.No.:E-BC-K022-M
Detection instrument: Microplate reader(530-570 nm,optimum wavelength: 550 nm)

Detection method: Colorimetric method

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Detection principle

Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

Performance characteristics

Synonyms	CuZn-SOD,Mn-SOD,T-SOD
Sample type	Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity	1.35 U/mL
Detection range	1.35-62 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 9.600%Average intra-assay CV: 5.100%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	Reagent 4: -20℃, others: 2-8℃
Valid period	12 months

Images

The activity of SOD was significantly changed between different superscripted letters. (P<0.05)

Y M Alharbi et al investigate the antioxidative and abtidiabetic effect of probiotic-enriched fermented camel milk combined with Salvia officinalis leaves extract. Superoxide dismutase (SOD) activity of rat blood was determined using SOD activity assay kit (E-BC-K022-M).

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Mouse serum	5-10
Rat serum	6-15
Urine	2-3
Human hydrothorax	2-3
10% Mouse liver tissue homogenate	100-200
10% Mouse brain tissue homogenate	20-30
10% Mouse kidney tissue homogenate	50-120
10% Rat kidney tissue homogenate	50-120
HepG2 cells (5.21mgprot/mL)	15-25
Cell culture supernatant	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

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The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

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DOI: 10.3390/foods11193048
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PMID: 36375957	Sample: larvae

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PMID: 35181488

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DOI: 10.1016/j.biopha.2020.110483
PMID: 32768965	Sample: Plasma,Tissue homogenate

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DRP1 promotes lactate utilization in KRAS‐mutant non‐small‐cell lung cancer cells

DOI: 10.1111/cas.14603
	Sample: Cell culture supernatant

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DOI: 10.1080/21655979.2022.2031769
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Brain and Behavior (2022) IF: 3.405

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DOI: 10.1002/brb3.2778
PMID: 36271663	Sample: Spinal cord injury (SCI) area

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PMID: 36152772	Sample: brain tissue

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DOI: 10.21037/atm-22-483
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DOI: 10.1038/s41598-020-63498-3
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PMID: 32848317	Sample: Serum

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