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Cyclooxygenase (COX) Activity Fluorometric Assay Kit

Cat.No.:E-BC-F050
Detection instrument: Fluorescence Microplate reader

Detection method: Fluorescence method

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96T

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Detection principle

Arachidonic acid generates prostaglandins under the catalysis of cyclooxygenase, and under the action of cofactor (heme), cyclooxygenase activates peroxidase activity, converting fluorescent red into fluorescent substance.

Performance characteristics

Sample type	animal tissue
Sensitivity	0.03 U/L
Detection range	0.03-9.66 U/L
Detection method	Fluorescence method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 5.0-7.0%Average intra-assay CV: 3.5-5.0%
Other reagents required	Normal saline (0.9% NaCl)
Storage	-20℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K135-M	Nitric Oxide (NO) Colorimetric Assay Kit (Nitrate Reductase Method)

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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