Current Position:Home>>Kits>>Metabolism Assays>> D-Lactic Acid/Lactate(LA)Colorimetric Assay Kit

D-Lactic Acid/Lactate(LA)Colorimetric Assay Kit

Cat.No.:E-BC-K002-M
Detection instrument: Microplate reader(520 nm-550 nm,optimum wavelength: 530 nm)

Detection method: Colorimetric method

To Purchase E-BC-K002-M

Size:

96T
48T

Price: $300

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A

Performance characteristics

Synonyms	D-LA
Sample type	Serum, plasma, animal tissue, saliva sample
Sensitivity	0.06 mmol/L
Detection range	0.06-8.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 7.700%Average intra-assay CV: 3.800%
Other instruments required	Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required	PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

Plasma D-LA level was significantly increased in VDN group, and reversed in SP treated group. (****P<0.0001)

Jianlong Yan et al investigates the beneficial effect of the short-chain fatty acid in intestinal microbiota remodeling. Plasma D-Lactate of rat was determined using D-lactate colorimetric assay kit (E-BC-K002-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.06-8.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	1
Human serum	1
Rat serum	1
Rat plasma	1
Mouse serum	1
Rabbit serum	1
10% Rat kidney tissue homogenate	2-3
10% Mouse brain tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Microbiome (2022) IF: 16.837
Beneficial effect of the short-chain fatty acid propionate on vascular calcification through intestinal microbiota remodelling

DOI: 10.1186/s40168-022-01390-0

Sample: plasma
International Journal of Nanomedicine (2023) IF: 7.033
Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A

DOI: 10.2147/IJN.S394544

Sample: blood
CANCER SCIENCE (2022) IF: 6.518
circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

DOI: 10.1111/cas.15351

Sample: AGS cell,HGC-27 cell
Pharmaceutics (2022) IF: 6.525
Two-in-One Nanoparticle Formulation to Deliver a Tyrosine Kinase Inhibitor and microRNA for Targeting Metabolic Reprogramming and Mitochondrial Dysfunction in Gastric Cancer

DOI: 10.3390/pharmaceutics14091759

PMID: 36145507
Sample: AGS cell
CELL PROLIFERATION (2022) IF: 6.831
Altered gut microbiota and metabolites profile are associated with reduced bone metabolism in ethanol-induced osteoporosis

DOI: 10.1111/cpr.13245

PMID: 35688648
Sample: serum
Frontiers in Endocrinology (2021) IF: 5.555
Elevated Levels of Circulating Biomarkers Related to Leaky Gut Syndrome and Bacterial Translocation Are Associated With Graves’ Disease

DOI: 10.3389/fendo.2021.796212

Sample: serum
Frontiers in Microbiology (2022) IF: 5.64
Trans-Anethole Alleviates Subclinical Necro-Haemorrhagic Enteritis-Induced Intestinal Barrier Dysfunction and Intestinal Inflammation in Broilers

DOI: 10.3389/fmicb.2022.831882

Sample: Serum
MOLECULAR NUTRITION & FOOD RESEARCH (2021) IF: 5.82
Daily Dose of Bovine Lactoferrin Prevents Ethanol-Induced Liver Injury and Death in Male Mice by Regulating Hepatic Alcohol Metabolism and Modulating Gut Microbiota

DOI: 10.1002/mnfr.202100253

PMID: 34331394
Sample: serum
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022) IF: 5.895
Marine Fish Protein Peptide Regulating Potassium Oxonate-Induced Intestinal Dysfunction in Hyperuricemia Rats Helps Alleviate Kidney Inflammation

DOI: 10.1021/acs.jafc.2c04017

Sample: serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Canagliflozin Modulates Hypoxia-Induced Metastasis, Angiogenesis and Glycolysis by Decreasing HIF-1α Protein Synthesis via AKT/mTOR Pathway

DOI: 10.3390/ijms222413336

Sample: HepG2 cell
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2023) IF: 3.094
Metabolic Phenotype Intricacies on Altered Glucose Metabolism of Breast Cancer Cells upon Glut-1 Inhibition and Mimic Hypoxia In Vitro

DOI: 10.1007/s12010-023-04373-5

Sample: SKBR-3 breast cancer cell
REPRODUCTION (2020) IF: 3.206
Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats

DOI: 10.1530/REP-20-0381

PMID: 33112813
Sample: testis
Clinical & Translational Oncology (2022) IF: 3.34
Identification of HAGHL as a novel metabolic oncogene regulating human colorectal cancer progression

DOI: 10.1007/s12094-022-03008-1

Sample: CRC tissue,HCT116 cell,LOVO cell
Biomedical Engineering Online (2021) IF: 2.819
Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression

DOI: 10.1186/s12938-021-00903-6

PMID: 34217312
Sample: Huh-8 cells
Cancer Management and Research (2020) IF: 2.886
CircSEC31A Promotes the Malignant Progression of Non-Small Cell Lung Cancer Through Regulating SEC31A Expression via Sponging miR-376a

DOI: 10.2147/CMAR.S280124

PMID: 33204164
Sample: Cell sample
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2022) IF: 2.945
CircCDC6 restrains tumor growth and glycolysis energy metabolism in colorectal cancer via regulating miR-3187-3p and downstream PRKAA2

DOI: 10.1007/s10863-022-09938-3

PMID: 35438362
Sample: SW480 cell,HCT116 cell

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

j***********sSubmitted [ Nov 29 2022 ]

Asked: has it been used in animal or human studies? if the answer is positive. Could you provide me the references about it. Thank you so much.

adminSubmitted [ Nov 29 2022 ]

Answered: For E-BC-K002-M, this kit can be used in animal or human studies. The references will be sent to you by email.

M**********dSubmitted [ Aug 30 2022 ]

Asked: Must I use 530 nm for the detection? I only have a microplate reader with 540 nm.

adminSubmitted [ Aug 30 2022 ]

Answered: 540 nm also works for this assay. Actually, the range between 520-550 nm is working, while 530 is the best choice with highest sensitivity.

D********dSubmitted [ Aug 05 2022 ]

Asked: Can I use lysis solution for cell sample preparation?

adminSubmitted [ Aug 05 2022 ]

Answered: It is not recommended to use lysate. Some components of lysate may interfere with the detection. Biochemical kits generally use the recommended homogenization medium for ultrasonication or mechanical homogenization.

... Show All Show Less

Cat. No	E-BC-K002-M
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha