L-Lactic Acid (LA) Colorimetric Assay Kit (E-BC-K044-M)
For research use only.
| Detection Principle | Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated. |
| Sample Type | Serum,Plasma,Cell culture supernatant,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (520-540 nm, optimum wavelength: 530 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.10 mmol/L |
| Detection Range | 0.12-7.0 mmol/L |
| Precision | inter-assay CV: 3.5% | intra-assay CV: 1.4% |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 2-5 |
| 10% Rat kidney tissue homogenate | 1-3 |
| 10% Rat brain tissue homogenate | 1 |
| HepG2 cell culture supernatant | 1 |
| HepG2 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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