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Cat.No.:E-BC-K761-M
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Synonyms | DBIL |
Sample type | Animal serum |
Sensitivity | 0.6 μmol/L |
Detection range | 0.6-50 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 35 min |
Precision | Average inter-assay CV: 8.600%Average intra-assay CV: 4% |
Other instruments required | Micropipettor, Vortex mixer, Centrifuge |
Storage | 2-8℃ |
Valid period | 12 months |
The concentration of DBIL was significantly reduced by decalactone. (*P<0.05, **P<0.01, ***P<0.001 vs. DEN)
H Y Shen et al investigate the chemoprotective effect of decalactone on hepatic cancer. Direct bilirubin (DBIL) in rat liver was determined using DBIL colorimetric assay kit (E-BC-K761-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.7-50 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Rat serum |
1 |
Mouse serum |
1 |
Rabbit serum |
1 |
Chicken serum |
1 |
Porcine serum |
1 |
Note: The diluent is normal saline (0.9% NaCl).
DOI: 10.5650/jos.ess22033 |
|
PMID: 35965085 |
Sample: serum |