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Direct Bilirubin (DBIL) Colorimetric Assay Kit

  • Cat.No.:E-BC-K761-M

  • Detection instrument: Microplate reader (565 nm)

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Detection principle

Bilirubin is one of the important components of bile. It is the degradation product of hemoglobin in various heme proteins under the action of a series of enzymes. It is important to the digestion and absorption of lipids and the formation of yellow distemper. Bilirubin comes in two forms: water-soluble and water-insoluble. Bilirubin has powerful antioxidant, anti-inflammatory and autoimmune properties. The concentration of bilirubin in human body is related to sex, drug intake, age and so on. Low serum bilirubin is directly related to diabetes, metabolic syndrome, cardiovascular disease and other pathological states. However, high bilirubin is indicative of hemolysis, jaundice, Gilbert syndrome, hepatitis, drug toxicity, and possible bile duct obstruction.

Performance characteristics

Synonyms DBIL
Sample type Animal serum
Sensitivity 0.6 μmol/L
Detection range 0.6-50 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 35 min
Precision Average inter-assay CV: 8.600%Average intra-assay CV: 4%
Other instruments required Micropipettor, Vortex mixer, Centrifuge
Storage 2-8℃
Valid period 12 months


The concentration of DBIL was significantly reduced by decalactone. (*P<0.05, **P<0.01, ***P<0.001 vs. DEN)

H Y Shen et al investigate the chemoprotective effect of decalactone on hepatic cancer. Direct bilirubin (DBIL) in rat liver was determined using DBIL colorimetric assay kit (E-BC-K761-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.7-50 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Rat serum


Mouse serum


Rabbit serum


Chicken serum


Porcine serum


Note: The diluent is normal saline (0.9% NaCl).


  1. Journal of Oleo Science (2022) IF: 1.628
    Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

    DOI: 10.5650/jos.ess22033

    PMID: 35965085

    Sample: serum


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