The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

General Information

Product Form	Powder
pH	7.2-7.4
D-Glucose	3151mg/L
HEPES	Negative
L-glutamine	2.5mM
NaHCO₃	Negative
Phenol red	8.1 mg/L
Sodium pyruvate	0.5mM
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	36 months

Notes

1. This product is for research use only.;
2. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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