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DMEM (High glucose), powder

  • Cat.No.:PM150210P

To Purchase PM150210P

  • 1×10L
  • 5×1L
  • 1×50L
Price: Inquire


DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

General Information

Product Form Powder
pH 7.2-7.4
D-Glucose 4500mg/L
HEPES negative
L-glutamine 4mM
NaHCO3 negative
Phenol red 15mg/L
Sodium pyruvate 1 mM
Storage 2-8℃ ,Shading Light
Shipping RT
Expiration date 36 months


1. This product is for research use only.;
2. It is necessary to pay attention to the aseptic operation and avoid the contamination.



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  • Q&A

A**aSubmitted [ Nov 24 2023 ]

Asked: How to prepar the media as a powder form?



adminSubmitted [ Nov 24 2023 ]

Answered: Taking the PM150210P-1L as an example: 1. The net weight of this product is 13.48 g, and 1L DMEM (High glucose) medium can be prepared. 2. Dissolve the DMEM (High glucose) powder with ultrapure water for cell culture. 3. Add 2.2g NaHCO3 and dissolve fully. 4. (Optional) Adjust PH with 1M NaOH solution or 1M HCl solution. 5. Add ultrapure water for cell culture to the final volume of 1L. 6. The prepared solution should be sterilized with 0.22 μm filters under aseptic conditions.


S***aSubmitted [ Jan 12 2021 ]

Asked: How much HEPES and NaHco3 should I add to 1L of the DMEM (High glucose, Catalog number: EP-CM-P0031) while preparing.



adminSubmitted [ Jan 12 2021 ]

Answered: Add 2.2g NaHCO3 and 5.95g HEPES to 1L of the DMEM (High glucose) powder and dissolve fully.

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