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EDTA Decalcification Solution

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      Catalog number:E-IR-R112

      Size:
      • 100mL
      • 500mL
      • 1000mL
      Qty:
      - +
      Price: $25

      Application: IHC

      Storage: Store at 2~8°C. Avoid direct sunlight. Valid for 12 months.

      Lead Time:Order now, ship in 3 daysWelcome to order from local distributors.

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      Introduction

      There are many hard calcium tissue in pathological experiments. The density between calcium and paraffin in tissue are different, and tissue containing calcium can’t be sliced directly. Bone tissue contains the most calcium and calcification may occur in other tissues forming calcified areas in the tissue, it needs to decalcify. Decalcification is an important step in making bone slices, especially the bone tissue to immunohistochemical stained.

      EDTA can combine with calcium in the outer layer of hydroxyapatite crystal to form soluble non-ionic compounds, and promote the outward transfer of bound calcium in the inner layer of the crystal. With the continuity, the hydroxyapatite crystal is gradually melted, and it can play a chelating role when the pH is neutral. This method is characterized by long decalcification time, less damage to bone tissue, better preservation of enzyme activity (alkaline phosphatase) and cell antigenicity. The slices can be used for histochemical and immunohistochemical analysis.


      Components

      Cat.

      Products

      100 mL

      500 mL

      1000 mL

      Storage

      E-IR-R112

      10% EDTA Decalcification Solution

      100 mL

      500 mL

      500 mL×2

      2~8°C

      Manual

       One Copy




      Experimental Procedure

      1. Cut the bone tissue to 0.5 cm × 0.3 cm × 0.2 cm pieces, wash with normal saline and put them into frozen Tissue Fixation Solution (It is recommended to use Elabscience® E-IR-R113) immediately and fix at 4°C for 12 h~24 h.

      2. Wash the bone pieces in 0.2 mol phosphate buffer.

      3. Immerse the bone pieces into the EDTA Decalcification Solution, decalcify at room temperature, and check the decalcification every day until the bone pieces are completely decalcified.

      Tips: Replace the EDTA Decalcification Solution every 4~5 days. After 3 times of replacement, replace the new EDTA Decalcification Solution every day.

      Please analyze according to the specific experiment. Finish the decalcification when the needles can penetrate into the bone.

      4. Wash the bone pieces with distilled water for 20 min.

      5. Routine dehydration procedures and embedding and slicing.


      Storage

      Store at 2~8°C. Avoid direct sunlight. Valid for 12 months.


      Cautions

      1. Sealed storage to prevent from pollution.

      2. This product is for research use only. It couldn’t be used for clinical diagnosis or treatment, food or medicine, and can’t be stored in residence.

      3. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

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