EHD2 Polyclonal Antibody
Price: $ 530
Price: $ 320
Price: $ 200
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:A549,U87-MG,HeLa,Mouse lung,Rat lung |
Dilution |
WB 1:1000-1:3000 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant fusion protein of human EHD2 (NP_055416.2). |
Abbre | EHD2 |
Synonyms | EHD2;PAST2 |
Swissprot | |
Calculated MW | 46kDa/61kDa |
Observed MW |
61kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cell membrane. Endosome membrane. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. |